Type: SPE Application #: 302940
Detection: The last extract is evaporated to dryness and the remainder can be dissolved in 100µl of HPLC mobile phase for normal-phase HPLC determination or it can be used for trimethylsilyl derivatization of th Conditions: 1. Sample application: Serum (100 μl) is applied on a Pasteur pipette filled with Chromabond XTR (filling height 4 cm), and after waiting 5 minutes, 200 μl methanol is added to break the lipid/protein bonds.
Elution: n-Hexane (5 ml) as eluant then yields the total lipid extract. This extract is evaporated to dryness.
2. Sample application: After evaporation of n-hexane the lipid extract is then further purified by chromatography on silica gel minicolumns made from Pasteur pipettes (filling height 4 cm) by applying all of the formerly mentioned lipid extract with 2x100μl of n-hexane onto the column.
Elution: after waiting 4 minutes the column is eluted using 4ml n-hexane-diethyl ether (5:1) Authors: Melchert H-U Pabel E Source: Robert Koch Institut Epidemiologie nicht über